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Low Evolutionary Diversification in a Widespread and Abundant Uncultured Protist (MAST-4) ArchiMer
Rodriguez-martinez, Raquel; Rocap, Gabrielle; Logares, Ramiro; Romac, Sarah; Massana, Ramon.
Recent culture-independent studies of marine planktonic protists have unveiled a large diversity at all phylogenetic scales and the existence of novel groups. MAST-4 represents one of these novel uncultured lineages, and it is composed of small (similar to 2 mu m) bacterivorous eukaryotes that are widely distributed in marine systems. MAST-4 accounts for a significant fraction of the marine heterotrophic flagellates at the global level, playing key roles in the marine ecological network. In this study, we investigated the diversity of MAST-4, aiming to assess its limits and structure. Using ribosomal DNA (rDNA) sequences obtained in this study (both pyrosequencing reads and clones with large rDNA operon coverage), complemented with GenBank sequences, we...
Tipo: Text Palavras-chave: MAST-4; Low evolutionary diversification; Uncultured protist; Pyrosequencing; ITS secondary structure.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00374/48506/48778.pdf
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